Leptin, MTS-tagged
Recombinant ovine MTS-leptin, one polypeptide chain containing 157 amino and (extra 10 amino acids of the membrane translocating sequence Val-Leu-Leu-Pro-Val-Leu-Leu-Ala-Ala-Pro) derived from Kaposi virus and additional Ala at N-terminus acids . The resulting molecular mass of is ~ 17.5 kDa, MTS-ovine leptin was purified by proprietary chromatographic techniques.
Product Specifications
Synonyms
Lep; ob; obese
NCBI Gene ID
443534
UniProt
G8BLB2
Accession Number
XP_027824581.2
Accession Number mRNA
XM_027968780.2
Reactivity
Ovine
Cross Reactivity
Ovine
Sequence
AAVLLPVLLAAPVPIRKVQDDTKTLIKTIVTRINDISHTQSVSSKQRVTGLDFIPGLHPLLSLSKMDQTLAIYQQILASLPSRNVIQISNDLENLRDLLHLLAASKSCPLPQVRALESLESLGVVLEASLYSTEVVALSRLQGSLQDMLRQLDLSPG
Assay Protocol
It is recommended to reconstitute the lyophilized MTS-ovine recombinant leptin in sterile 0.4% NaHCO3 adjusted, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Endotoxin
< 0.1 ng/µg of protein (< 1EU/µg)
Purity
> 98.0% as determined by Gel filtration and SDS-PAGE gel.
Bioactivity
MTS-ovine recombinant leptin is fully biologically active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. In vivo experiments has shown potency equal but not higher that non tagged ovine leptin (A. Gertler, unpublished data) .
Length
157
Form
Lyophilized
Reconstitution
0.4% NaHCO3, pH 8-9
Molecular Weight
17.5 kDa
Host or Source
E. coli
N Terminal Sequence
AAVLLP
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
11768126 3x 1.0µg DNA
ALG8 CRISPRa sgRNA lentivector (set of three targets)(Rat)
Sign In for Pricing
View Details 137970 1 mL
Fibrinogen (Coagulation Factor I) (Not for Export EU) discontinued
Sign In for Pricing
View Details PC1003028-01 100 mg
2-Methoxy-5-methyl-3- (trifluoromethyl) pyridine
Sign In for Pricing
View Details