Anti-NFkB-p65 (Phospho-Thr435) Antibody

Rabbit Polyclonal Antibody specific to NFkB-p65 (Phospho-Thr435)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-NFkB-p65 (Phospho-Thr435) Rabbit Polyclonal Antibody

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Peptide sequence that includes phosphorylation site of threonine 435 (E-G-T(p)-L-S) derived from human NFkappaB-p65 and conjugated to KLH.

Target

NFkB-p65 (Phospho-Thr435)

Clonality

Polyclonal

Type

Antibody

Applications

WB, IHC, IF

Field of Research

Phosphospecific Antibodies

Purification Method

Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Additionnal Information

Western blotting: use at dilution of 1:500-1:1,000. A band of ~65kDa is detected. Immunohistochemistry: use at dilution of 1:50-1:100. Immunofluorescence: use at dilution of 1:100-1:200. These are recommended working dilutions. End user should determine optimal dilutions for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody detects endogenous human, mouse, and rat NFkappaB-p65 only when phosphorylated at threonine 435.

Formulation

PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Buffer pH

pH 7.4

Target Background

NFkappaB is a transcription factor found in almost all cell types and is involved in many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NFkappaB is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. such as p65-p50 and p65-c-Rel complexes. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression.