Biotinylated Human DLL4 Protein, Fc, Avitag™, premium grade

Delta-like protein 4 (DLL4) is also known as Drosophila Delta homolog 4 (Delta4), which contains one DSL domain and eight EGF-like domains. DLL4 is expressed in vascular endothelium. DLL4 is involved in the Notch signaling pathway as Notch ligand, which can activates NOTCH1 and NOTCH4. DLL4 is involved in angiogenesis and negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. DLL4 can bind to Notch-1 and Notch-4.

Product Specifications

Background

Specifications

This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 82.4 kDa. The protein migrates as 98 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

NP_061947.1

Expression Region

Ser 27 - Pro 524

Host

HEK293

Target

DLL4

Conjugation

Biotin-labeled

Tag

C-Fc & C-Avi

Source

Human

Stability

-20°C to -70°C for 24 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

0.01 EU per μg

Purity

95%

Bioactivity

Immobilized Human NOTCH1, Fc Tag (Cat. No. NO1-H5255) at 5 μg/mL (100 μL/well) can bind Biotinylated Human DLL4 Protein, Fc,Avitag, premium grade (Cat. No. DL4-H82F7) with a linear range of 2-16 ng/mL (QC tested).