Recombinant SARS-CoV-2 Spike Glycoprotein

Attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein (PubMed:32142651, PubMed:32221306, PubMed:32075877, PubMed:32155444) . Binding to host NRP1 and NRP2 via C-terminal polybasic sequence enhances virion entry into host cell (PubMed:33082294, PubMed:33082293) . This interaction may explain virus tropism of human olfactory epithelium cells, which express high level of NRP1 and NRP2 but low level of ACE2 (PubMed:33082293) . The stalk domain of S contains three hinges, giving the head unexpected orientational freedom (PubMed:32817270) . Uses human TMPRSS2 for priming in human lung cells which is an essential step for viral entry (PubMed:32142651) . Can be alternatively processed by host furin (PubMed:32362314) . Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membrane fusion within endosomes.

RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNF

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

1. Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 ug/ml can bind SARS-CoV-2-S Antibody , the EC50 of SARS-CoV-2-S1-RBD protein is 19.60-39.42 ng/ml. 2. Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 5 ug/ml can bind human ACE2 , the EC50 of SARS-CoV-2-S1-RBD protein is 31.80 - 44.69 ng/ml. 3. Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 ug/ml can bind SARS-CoV-2-S Antibody , the EC50 of SARS-CoV-2-S1-RBD protein is 13.48-19.50 ng/ml. 4. SARS-CoV-2 Spike protein RBD his/sumostar tag captured on COOH chip can bind Human ACE2 protein Fc tag with an affinity constant of 100 nM as detected by LSPR Assay. 5. SARS-CoV-2 Spike protein RBD His/Sumostar Tag captured on COOH chip can bind SARS-CoV-2 Spike RBD Nanobody with an affinity constant of 28.2nM as detected by LSPR Assay.