Rat TSH (Thyroid Stimulating Hormone) ELISA Kit

Product Specifications

Abbreviation

TSH

UniProt

P11962&P04652

Cross Reactivity

This kit recognizes Rat TSH in samples. No significant cross-reactivity or interference between Rat TSH and analogues was observed

Target

Thyrotropin

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Metabolism; Neuroscience; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TSH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TSH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TSH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TSH. You can calculate the concentration of Rat TSH in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

1.25-80 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.75 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S004896972100262X

Citation 02

https://www.sciencedirect.com/science/article/pii/S0045653521021081

Citation 03

https://www.sciencedirect.com/science/article/pii/S2001037020304761

Citation 04

https://jme.bioscientifica.com/view/journals/jme/67/3/JME-21-0095.xml

Citation 05

http://dmd.aspetjournals.org/content/46/6/786.abstract

Citation 06

https://bmccomplementmedtherapies.biomedcentral.com/articles/10.1186/s12906-022-03507-2

Citation 07

https://link.springer.com/article/10.1007/s12011-022-03204-8

Citation 08

https://www.sciencedirect.com/science/article/pii/S0753332217349375

Citation 09

https://www.sciencedirect.com/science/article/pii/S0166432822003503

Citation 10

https://www.hindawi.com/journals/bmri/2021/6681491/

Citation 11

https://jps.biomedcentral.com/articles/10.1007/s12576-018-0595-7

Citation 12

https://journals.sagepub.com/doi/abs/10.1177/1091581820986073

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat TSH concentrations in serum, plasma and other biological fluids.

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