STAT4 ELISA Kit (Human)
Product Specifications
Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
Signal transducer and activator of transcription 4
Gene Aliases
Signal transducer and activator of transcription 4; signal transducer and activator of transcription 4 variant 3; SLEB11.
Gene ID
6775
Accession Number
NP_001230764
Reactivity
Homo sapiens, Human
Target
Signal transducer and activator of transcription 4
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology STAT4 ELISA Kit (Human) (OKEH07174) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for STAT4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for STAT4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample STAT4 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into matrix = 108.3%
Sensitivity
0.168 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Signal transducer and activator of transcription 4
NCBI Gene Symbol
STAT4
Protein Name
Q14765
Gene Name URL
STAT4
Nucleotide Accession Number
NM_001243835
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