AKR7A3 ELISA Kit (Rat)
Product Specifications
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Aldo-keto reductase family 7 member A3
Gene Aliases
Afar; AFB1-AR; aflatoxin B1 aldehyde reductase member 1; aflatoxin B1 aldehyde reductase member 3; Akr7a1; aldo-keto reductase family 7, member A3 (aflatoxin aldehyde reductase) ; rAFAR1.
Gene ID
26760
Accession Number
NP_001298064
Reactivity
Rat, Rattus norvegicus
Target
Aflatoxin B1 aldehyde reductase member 3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Akr7a3 ELISA Kit (Rat) (OKEH06485) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Akr7a3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Akr7a3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Akr7a3 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 101%
Sensitivity
0.415 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Aflatoxin B1 aldehyde reductase member 3
NCBI Gene Symbol
Akr7a3
Protein Name
P38918
Gene Name URL
Akr7a3
Nucleotide Accession Number
NM_001311135
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog