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TRAF6 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-Assay Precision -

CAS Number

7732-18-5

Gene Name

TNF receptor-associated factor 6

Gene Aliases

2310003F17Rik; AI851288; C630032O20Rik; E3 ubiquitin-protein ligase TRAF6; RING-type E3 ubiquitin transferase TRAF6; TNF receptor-associated factor 6.

Gene ID

22034

Accession Number

NP_001290202.1

Reactivity

Mouse, Mus musculus

Target

TNF receptor-associated factor 6

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Traf6 ELISA Kit (Mouse) (OKEH05019) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Traf6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Traf6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Traf6 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 83%

Sensitivity

0.082 ng/mL

Reconstitution

Store as indicated in product manual.

Specificity

Natural and recombinant Mouse TNF receptor-associated factor 6

Protein Length

530

NCBI Gene Symbol

Traf6

Protein Name

P70196

Gene Name URL

Traf6

Nucleotide Accession Number

NM_001303273.1

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