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MUM1 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

PWWP domain containing 3A, DNA repair factor

Gene Aliases

EXPAND1; HSPC211; melanoma associated antigen (mutated) 1; melanoma ubiquitous mutated protein; MUM1; MUM-1; mutated melanoma-associated antigen 1; protein expandere; PWWP domain-containing DNA repair factor 3A; PWWP domain-containing protein MUM1.

Gene ID

84939

Accession Number

NP_116242.2

Reactivity

Homo sapiens, Human

Target

PWWP domain-containing protein MUM1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology MUM1 ELISA Kit (Human) (OKEH04636) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MUM1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for MUM1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MUM1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

78-5000pg/mL

Sensitivity

32 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

79 KDa

Specificity

Natural and recombinant Human PWWP domain-containing protein MUM1

Protein Length

710

NCBI Gene Symbol

PWWP3A

Protein Name

Q2TAK8

Gene Name URL

PWWP3A

Nucleotide Accession Number

NM_032853.3

Frequently Asked Questions

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