Cxcl2 ELISA Kit (Mouse)
Product Specifications
CAS Number
7732-18-5
Gene Name
Chemokine (C-X-C motif) ligand 2
Gene Aliases
CINC; CINC-2a; C-X-C motif chemokine 2; Gr; Gro2; GROb; macrophage inflammatory protein 2; Mgsa; Mgsa-b; Mi; MIP; Mip2; MIP-2; MIP-2a; Scy; Scyb; Scyb2; small inducible cytokine subfamily, member 2.
Gene ID
20310
Accession Number
NP_033166.1
Reactivity
Mouse, Mus musculus
Target
C-X-C motif chemokine 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology Cxcl2 ELISA Kit (Mouse) (OKEH04515) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cxcl2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Cxcl2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cxcl2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids
Detection Range
0.312-20ng/mL
Sensitivity
0.166 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
10 KDa
Specificity
Natural and recombinant Mouse C-X-C motif chemokine 2
Protein Length
100
NCBI Gene Symbol
Cxcl2
Protein Name
P10889
Gene Name URL
Cxcl2
Nucleotide Accession Number
NM_009140.2
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