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PGA3 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Pepsinogen A3

Gene Aliases

Pepsin A-3; pepsinogen 3, group I (pepsinogen A) ; pepsinogen-3.

Gene ID

643834

Accession Number

NP_001073275.1

Reactivity

Homo sapiens, Human

Target

Pepsin A-3

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology PGA3 ELISA Kit (Human) (OKEH03169) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PGA3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for PGA3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PGA3 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

1.56-100ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%

Sensitivity

< 1.56 ng/mL.

Reconstitution

Store as indicated in product manual.

Molecular Weight

42 kDa

Specificity

This assay recognizes recombinant and natural human Pepsin A. No significant cross-reactivity or interference was observed.

Protein Length

388

NCBI Gene Symbol

PGA3

Protein Name

P0DJD8

Gene Name URL

PGA3

Nucleotide Accession Number

NM_001079807.3

Frequently Asked Questions

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