What is the catalog number for DEFB14 ELISA Kit (Mouse)?

The catalog number for DEFB14 ELISA Kit (Mouse) is OKEH03151-96W.

What sizes are available for DEFB14 ELISA Kit (Mouse)?

DEFB14 ELISA Kit (Mouse) is available in the following size(s): 96 Well.

Is DEFB14 ELISA Kit (Mouse) in stock?

DEFB14 ELISA Kit (Mouse) is currently made to order / not held in immediate stock. Contact us for lead time.

Can I request a Safety Data Sheet (SDS) for this product?

Yes, safety data sheets are available for hazardous or chemically classified products and can be provided on request or downloaded from the product page.

Can this product be used for co-immunoprecipitation (Co-IP)?

Suitability for Co-IP depends on the specific antibody and is noted under validated applications on the datasheet. If it isn’t listed, contact our technical support team for guidance based on your target and sample type.

Is this kit compatible with automated liquid handling platforms?

Automation compatibility varies by kit and isn’t always listed on the datasheet. Contact our technical support team with details of your platform so we can confirm with the manufacturer.

DEFB14 ELISA Kit (Mouse)

Selected Variant

OKEH03151-96W

Size: 96 Well

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Defensin beta 14

Gene Aliases

BD-14; beta-defensin 14; Defensin, beta 14.

Gene ID

244332

Accession Number

NP_898847.1

Reactivity

Mouse, Mus musculus

Target

Beta-defensin 14

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology DEFB14 ELISA Kit (Mouse) (OKEH03151) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Defb14 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Defb14 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Defb14 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 107%

Sensitivity

0.091 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

77 kDa

Specificity

Natural and recombinant Mouse Beta-defensin 14

Protein Length

NCBI Gene Symbol

Defb14

Protein Name

Q7TNV9

Gene Name URL

Defb14

Nucleotide Accession Number

NM_183026.2

Price

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