SARS-CoV-2 Nucleocapsid protein (T205I), His Tag

SARS-CoV-2 Nucleocapsid protein (T205I), His Tag (NUN-C52Hd) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 419 (Accession # QHO62115.1 (T205I)). The nucleocapsid protein is consisted of the N-terminal RNA-binding domain (NTD) and the C-terminal dimerization domain (CTD), divided by a central serine/arginine-rich (SR)-linker responsible for phosphorylation. The mutation T205I was identified in the SARS-CoV-2 Beta (Pango Lineage: B.1.351) and Mu (Pango Lineage: B.1.621) variants (data retrieved from https://pubmed.ncbi.nlm.nih.gov/33095454/).

Product Specifications

Background

Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 47.3 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Expression Region

Met 1 - Ala 419

Host

HEK 293

Target

Nucleocapsid protein

Conjugation

Unconjugated

Tag

C-6×His

Source

SARS-CoV-2

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized SARS-CoV-2 Nucleocapsid protein (T205I), His Tag (Cat. No. NUN-C52Hd) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Nucleocapsid Antibody, Chimeric mAb, Human IgG1 with a linear range of 0.2-1 ng/mL (QC tested).