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HBA1 ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

Hemoglobin alpha, adult chain 1, hemoglobin alpha, adult chain 2

Gene Aliases

2-alpha globin;2-alpha-1 globin; alpha-1/2-globin; Glnc2; Glnc3; globin c2; globin c3; Hba1; Hba2; Hba-a1; Hba-a2; HBAM; hemoglobin alpha 1 chain; hemoglobin alpha 2 chain; hemoglobin alpha, adult chain 2; hemoglobin alpha-1/2 chain; hemoglobin subunit alpha-1/2; hemoglobin, alpha 1; hemoglobin, alpha 2.

Gene ID

25632, 360504

Accession Number

NP_001007723.1

Reactivity

Rat, Rattus norvegicus

Target

Hemoglobin subunit alpha-1/2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology HBA1 ELISA Kit (Rat) (OKEH03135) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hba1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Hba1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hba1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

3.12-200ng/mL

Sensitivity

1.59 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

15 kDa

Specificity

Natural and recombinant Rat Hemoglobin subunit alpha-1/2

Protein Length

142

NCBI Gene Symbol

Hba-a1, Hba-a2

Protein Name

P01946

Gene Name URL

Hba-a1, Hba-a2

Nucleotide Accession Number

NM_001007722.1

Frequently Asked Questions

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