Dihydrotestosterone ELISA Kit

Dihydrotestosterone (5alpha-Dihydrotestosterone, commonly abbreviated to DHT) is an androgen or male sex hormone. The enzyme 5alpha-reductase synthesises DHT in the prostate, testes, hair follicles, and adrenal glands. This enzyme reduces the 4,5 double-bond of the hormone testosterone.

Aviva Systems Biology Dihydrotestosterone ELISA Kit (OKEH02531) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Dihydrotestosterone antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Dihydrotestosterone and incubated. The Dihydrotestosterone found in the sample or standards competes with the biotinylated Dihydrotestosterone for limited binding sites on the immobilized anti-Dihydrotestosterone antibody. Excess unbound biotinylated Dihydrotestosterone and sample or standard Dihydrotestosterone is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Dihydrotestosterone captured in the well and inversely proportional to the amount of Dihydrotestosterone which was contained in the sample or standard.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol