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BCL11B ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

BAF chromatin remodeling complex subunit BCL11B

Gene Aliases

ATL1; ATL1-alpha; ATL1-beta; ATL1-delta; ATL1-gamma; B cell CLL/lymphoma 11B; B-cell CLL/lymphoma 11B; B-cell CLL/lymphoma 11B (zinc finger protein) ; B-cell CLL/lymphoma 11B/T-cell receptor delta constant region fusion protein; B-cell lymphoma/leukaemia 11B; B-cell lymphoma/leukemia 11B; BCL-11B; BCL11B, BAF complex component; BCL11B/TRDC fusion; COUP-TF-interacting protein 2; CTIP2; CTIP-2; hRit1; hRIT1-alpha; IDDFSTA; IMD49; radiation-induced tumor suppressor gene 1 protein; RIT1; SMARCM2; zinc finger protein hRit1 alpha; ZNF856B.

Gene ID

64919

Accession Number

NP_001269166.1

Reactivity

Homo sapiens, Human

Target

B-cell lymphoma/leukemia 11B

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology BCL11B ELISA Kit (Human) (OKEH02400) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BCL11B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BCL11B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BCL11B captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%

Sensitivity

23 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

96 kDa

Specificity

Natural and recombinant Human B-cell lymphoma/leukemia 11B

Protein Length

894

NCBI Gene Symbol

BCL11B

Protein Name

Q9C0K0

Gene Name URL

BCL11B

Nucleotide Accession Number

NM_001282237.1

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