BCL11B ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
BAF chromatin remodeling complex subunit BCL11B
Gene Aliases
ATL1; ATL1-alpha; ATL1-beta; ATL1-delta; ATL1-gamma; B cell CLL/lymphoma 11B; B-cell CLL/lymphoma 11B; B-cell CLL/lymphoma 11B (zinc finger protein) ; B-cell CLL/lymphoma 11B/T-cell receptor delta constant region fusion protein; B-cell lymphoma/leukaemia 11B; B-cell lymphoma/leukemia 11B; BCL-11B; BCL11B, BAF complex component; BCL11B/TRDC fusion; COUP-TF-interacting protein 2; CTIP2; CTIP-2; hRit1; hRIT1-alpha; IDDFSTA; IMD49; radiation-induced tumor suppressor gene 1 protein; RIT1; SMARCM2; zinc finger protein hRit1 alpha; ZNF856B.
Gene ID
64919
Accession Number
NP_001269166.1
Reactivity
Homo sapiens, Human
Target
B-cell lymphoma/leukemia 11B
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology BCL11B ELISA Kit (Human) (OKEH02400) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BCL11B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BCL11B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BCL11B captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
Sensitivity
23 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
96 kDa
Specificity
Natural and recombinant Human B-cell lymphoma/leukemia 11B
Protein Length
894
NCBI Gene Symbol
BCL11B
Protein Name
Q9C0K0
Gene Name URL
BCL11B
Nucleotide Accession Number
NM_001282237.1
Frequently Asked Questions
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