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Goat Rat IgG1 IgG2a IgG2b IgG2c (Fc specific) Antibody

Purified IgG fraction of polyclonal goat antiserum to rat IgG, Fc specific. As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user's own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Typical working dilutions in histochemistry are usually between 1:50 and 1:250; in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5,000.

Product Specifications

Reactivity

Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Source

Purified normal IgG, including all subclasses, isolated from pooled rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Field of Research

Cell Biology

Form

Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) . No preservative added, as it may interfere with the antibody activity.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Host or Source

Goat

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