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AMELX ELISA Kit (Mouse)

Selected Variant

OKEH02336-96W

Size: 96 Tests

Product Specifications

CAS Number

7732-18-5

Gene Name

Amelogenin, X-linked

Gene Aliases

ALGN; Am; Amel; amelogenin X chromosome; amelogenin, X isoform; Amg; AMGL; AMGX; enamel matrix protein; leucine-rich amelogenin peptide; LRAP; Rgsc8; Rgsc888.

Gene ID

11704

Accession Number

NP_001075447.1

Reactivity

Mouse, Mus musculus

Target

Amelogenin, X isoform

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology AMELX ELISA Kit (Mouse) (OKEH02336) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AMELX has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AMELX is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AMELX captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%

Sensitivity

0.079 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

23 kDa

Specificity

Natural and recombinant Mouse Amelogenin, X isoform

Protein Length

210

NCBI Gene Symbol

Amelx

Protein Name

P63277

Gene Name URL

Amelx

Nucleotide Accession Number

NM_001081978.2

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