Mouse ICAM-1 PicoKine® Quick ELISA Kit

Intercellular adhesion molecule-1 (ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated (LFA) antigens, a beta 2 leukocyte integrin. The normal function of human intercellular adhesion molecule-1 (ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen (LFA-1) or macrophage-1 antigen (Mac-1) . ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines. ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses. The standard product used in this kit is produced by recombinant gene expression, which is a dimeric protein linked with disulfide bonds. The single chain is Gln28-Asn 485. It has 457 amino acids with the molecular mass of 76.8kDa. As a result of glycosylation, the molecular mass of 110-130KDa is revealed by SDS-PAGE.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 354 362 357 334 351 10.63 3% Sample 2 1378 1386 1353 1604 1430 101.05 7% Sample 3 4978 4752 4911 4804 4861 88.48 1.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 354 362 357 334 351 10.63 3% Sample 1 354 362 357 334 351 10.63 3% Sample 2 1378 1386 1353 1604 1430 101.05 7% Sample 2 1378 1386 1353 1604 1430 101.05 7% Sample 3 4978 4752 4911 4804 4861 88.48 1.8% Sample 3 4978 4752 4911 4804 4861 88.48 1.8%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 354 1378 4978 361 1458 5187 Standard deviation 21.94 62.01 383.19 26.2%7 83.1 414.96 CV (%) 6.2% 4.5% 6.4% 7% 5.7% 8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 354 1378 4978 361 1458 5187 Standard deviation 21.94 62.01 383.19 26.2%7 83.1 414.96 CV (%) 6.2% 4.5% 6.4% 7% 5.7% 8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 354 1378 4978 361 1458 5187 Mean (pg/ml) 354 1378 4978 361 1458 5187 Standard deviation 21.94 62.01 383.19 26.2%7 83.1 414.96 Standard deviation 21.94 62.01 383.19 26.2%7 83.1 414.96 CV (%) 6.2% 4.5% 6.4% 7% 5.7% 8% CV (%) 6.2% 4.5% 6.4% 7% 5.7% 8%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.026 0.076 0.139 0.243 0.526 0.986 1.761 2.061 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.026 0.076 0.139 0.243 0.526 0.986 1.761 2.061 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.026 0.076 0.139 0.243 0.526 0.986 1.761 2.061 O.D. 0.026 0.076 0.139 0.243 0.526 0.986 1.761 2.061

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.