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ABCC6 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

ATP binding cassette subfamily C member 6

Gene Aliases

ABC34; anthracycline resistance-associated protein; ARA; ATP-binding cassette sub-family C member 6; ATP-binding cassette, sub-family C (CFTR/MRP), member 6; EST349056; GACI2; MLP1; MOATE; MOAT-E; MRP6; multidrug resistance-associated protein 6; multi-specific organic anion transporter E; PXE; PXE1; URG7; URG7 protein.

Gene ID

368

Accession Number

NP_001072996.1

Reactivity

Homo sapiens, Human

Target

Multidrug resistance-associated protein 6

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology ABCC6 ELISA Kit (Human) (OKEH02082) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ABCC6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ABCC6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ABCC6 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 112%

Sensitivity

21 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

165 kDa

Specificity

Natural and recombinant Human Multidrug resistance-associated protein 6

Protein Length

1503

NCBI Gene Symbol

ABCC6

Protein Name

O95255

Gene Name URL

ABCC6

Nucleotide Accession Number

NM_001079528.3

Frequently Asked Questions

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