ABCC6 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
ATP binding cassette subfamily C member 6
Gene Aliases
ABC34; anthracycline resistance-associated protein; ARA; ATP-binding cassette sub-family C member 6; ATP-binding cassette, sub-family C (CFTR/MRP), member 6; EST349056; GACI2; MLP1; MOATE; MOAT-E; MRP6; multidrug resistance-associated protein 6; multi-specific organic anion transporter E; PXE; PXE1; URG7; URG7 protein.
Gene ID
368
Accession Number
NP_001072996.1
Reactivity
Homo sapiens, Human
Target
Multidrug resistance-associated protein 6
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ABCC6 ELISA Kit (Human) (OKEH02082) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ABCC6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ABCC6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ABCC6 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 112%
Sensitivity
21 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
165 kDa
Specificity
Natural and recombinant Human Multidrug resistance-associated protein 6
Protein Length
1503
NCBI Gene Symbol
ABCC6
Protein Name
O95255
Gene Name URL
ABCC6
Nucleotide Accession Number
NM_001079528.3
Frequently Asked Questions
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