What is the catalog number for TMSB4X ELISA Kit (Mouse)?

The catalog number for TMSB4X ELISA Kit (Mouse) is OKEH01277-96W.

What sizes are available for TMSB4X ELISA Kit (Mouse)?

TMSB4X ELISA Kit (Mouse) is available in the following size(s): 96 Well.

Is TMSB4X ELISA Kit (Mouse) in stock?

TMSB4X ELISA Kit (Mouse) is currently made to order / not held in immediate stock. Contact us for lead time.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.

What happens if this product doesn’t perform as expected in my experiment?

Send us the catalogue number, product name, lot number, and a summary of your results. Our technical support team will investigate and, where needed, involve the manufacturer. This is covered under our guarantee provided the product was used before its expiry date and stored correctly.

Is this product classed as hazardous or restricted for shipping?

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TMSB4X ELISA Kit (Mouse)

Selected Variant

OKEH01277-96W

Size: 96 Well

Product Specifications

CAS Number

7732-18-5

Gene Name

Thymosin, beta 4, X chromosome

Gene Aliases

Prothymosin beta 4; Ptmb; Ptmb4; t beta 4; Tb4; Tbeta; Tbeta4; thymosin beta-4.

Gene ID

19241

Accession Number

NP_067253.1

Reactivity

Mouse, Mus musculus

Target

Thymosin beta-4

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology TMSB4X ELISA Kit (Mouse) (OKEH01277) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-mouse TMSB4X antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated TMSB4X and incubated. The TMSB4X found in the sample or standards competes with the biotinylated TMSB4X for limited binding sites on the immobilized anti-mouse TMSB4X antibody. Excess unbound biotinylated TMSB4X and sample or standard TMSB4X is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated TMSB4X captured in the well and inversely proportional to the amount of TMSB4X which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.312-20ng/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%

Sensitivity

0.168 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

6 kDa

Specificity

Natural and recombinant Mouse Thymosin beta-4

Protein Length

NCBI Gene Symbol

Tmsb4x

Protein Name

P20065

Gene Name URL

Tmsb4x

Nucleotide Accession Number

NM_021278.2

Price

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