Mouse LIF ELISA Kit PicoKine®

Leukemia inhibitory factor, or LIF, is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. The LIF was mapped gene to 22q11-q12.2 by Southern analysis of a series of mouse/human somatic cell hybrids and by in situ hybridization to the chromosomes of 2 normal males and some individuals with chromosomal rearrangements. The gene maps between the Philadelphia translocation BCR1 and the breakpoint of the translocation in cell line GM2324 at 22q12.2. LIF derives its name from its ability to induce the terminal differentiation of myeloid leukemic cells, thus preventing their continued growth. Other properties attributed to the cytokine include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 16 15 16 15 15 0.5 3.3% Sample 2 70 65 74 67 69 3.39 4.9% Sample 3 272 305 287 293 289 11.88 4.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 16 15 16 15 15 0.5 3.3% Sample 1 16 15 16 15 15 0.5 3.3% Sample 2 70 65 74 67 69 3.39 4.9% Sample 2 70 65 74 67 69 3.39 4.9% Sample 3 272 305 287 293 289 11.88 4.1% Sample 3 272 305 287 293 289 11.88 4.1%

Cancer, Cancer Susceptibility, Cell Biology, Cell Cycle, Cell Differentiation, Cytokines, Epigenetics and Nuclear Signaling, Immunology, Innate Immunity, Neurogenesis, Neurology Process, Neuroscience, Transcription, Tumor Suppressors

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 16 70 272 15 76 278 Standard deviation 0.7 3.01 19.58 0.72 3.34 24.74 CV (%) 4.4% 4.3% 7.2% 4.8% 4.4% 8.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 16 70 272 15 76 278 Standard deviation 0.7 3.01 19.58 0.72 3.34 24.74 CV (%) 4.4% 4.3% 7.2% 4.8% 4.4% 8.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 16 70 272 15 76 278 Mean (pg/ml) 16 70 272 15 76 278 Standard deviation 0.7 3.01 19.58 0.72 3.34 24.74 Standard deviation 0.7 3.01 19.58 0.72 3.34 24.74 CV (%) 4.4% 4.3% 7.2% 4.8% 4.4% 8.9% CV (%) 4.4% 4.3% 7.2% 4.8% 4.4% 8.9%

LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.029 0.071 0.131 0.265 0.507 0.837 1.315 1.81 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.029 0.071 0.131 0.265 0.507 0.837 1.315 1.81 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.029 0.071 0.131 0.265 0.507 0.837 1.315 1.81 O.D. 0.029 0.071 0.131 0.265 0.507 0.837 1.315 1.81

LIF is mostly contained as inactive form in samples, please activate it before assay. Don't activate recombinant LIF. Solution A: 1N HCI: add 8.33ml of 12N HCI into 91.67ml of H2O. Solution B: 1.2N NaOH/0.5M HEPES: add 12ml of 10N NaOH and 11.9g HEPES into 75ml of H2O, add H2O to adjust volume to 100ml.

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.