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REG1A ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

Regenerating family member 1 alpha

Gene Aliases

ICRF; islet cells regeneration factor; islet of Langerhans regenerating protein; LITHOST; lithostathine; pancreatic stone protein; pancreatic thread protein; PSP; PTP; RATLITHOST; RATRGPI; Reg; Reg1; regenerating islet-derived 1 alpha; regenerating islet-derived mouse homolog 1; regeneration protein, lithostatin, pancreatic stone protein; Rgp1; RGPI.

Gene ID

24714

Accession Number

NP_036773.1

Reactivity

Rat, Rattus norvegicus

Target

Lithostathine

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology REG1A ELISA Kit (Rat) (OKEH01263) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for REG1A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for REG1A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample REG1A captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

78-5000pg/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 90%

Sensitivity

39.7 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

19 kDa

Specificity

Natural and recombinant Rat Lithostathine

Protein Length

165

NCBI Gene Symbol

Reg1a

Protein Name

P10758

Gene Name URL

Reg1a

Nucleotide Accession Number

NM_012641.1

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