DHEA-S (human) ELISA Kit

DHEA-S (Dehydroepiandrosterone sulfate) is a metabolite of DHEA (Dehydroepiandrosterone). It is more specific product of the adrenals and measurements of this steroid is widely intended in clinical practice. The clinical importance of plasma assays of DHEA-S is associated with the diagnosis of adrenal hyperplasia and differential diagnosis of hirsutism. BioVision’s human DHEA-S kit is based on the principle of competitive binding between DHEA-S in the sample and HRP conjugated DHEA-S for a constant amount of anti-DHEA-S antibody. In the first incubation, Goat-anti-Rabbit-IgG coated wells are incubated with DHEA-S Standards, samples, DHEA-S Enzyme (HRP) reagent and anti-DHEA-S Antibody reagent, at room temperature. During the incubation, HRP labeled DHEA-S competes with the endogenous DHEAS in the standard and sample, for a fixed number of binding sites of the DHEA-S antibody, while simultaneously the Anti DHEA-S antibody binds to the immobilized secondary antibody. Thus, the amount of DHEA-S HRP conjugate immunologically bound to the well progressively decreases as the concentration of DHEA-S in the sample increases. Unbound DHEA-S HRP conjugate is removed and wells are washed. Next, TMB Reagent is added and incubated at room temperature, resulting in the development of blue color. The color development is stopped with addition of stop solution, and absorbance is spectrophotometrically measured at 450 nm. A standard curve is prepared relating color intensity to the concentration of DHEA-S. The sensitivity of this ELISA test is 0.1 μg/ml.