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NARG2 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Interactor of little elongation complex ELL subunit 2

Gene Aliases

BRCC1; breast cancer cell 1; interactor of little elongator complex ELL subunit 2; little elongation complex subunit 2; NARG2; NMDA receptor regulated 2; NMDA receptor-regulated gene 2; NMDA receptor-regulated protein 2.

Gene ID

79664

Accession Number

NP_001018099.1

Reactivity

Homo sapiens, Human

Target

Little elongation complex subunit 2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology NARG2 ELISA Kit (Human) (OKEH01118) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NARG2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NARG2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NARG2 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

15.6-1000pg/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 88%

Sensitivity

10 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

110 kDa

Specificity

Natural and recombinant Human NMDA receptor-regulated protein 2

Protein Length

982

NCBI Gene Symbol

ICE2

Protein Name

Q659A1

Gene Name URL

ICE2

Nucleotide Accession Number

NM_001018089.2

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