What is the catalog number for ARSB ELISA Kit (Human)?

The catalog number for ARSB ELISA Kit (Human) is OKDD00133-96W.

What sizes are available for ARSB ELISA Kit (Human)?

ARSB ELISA Kit (Human) is available in the following size(s): 96 Tests.

Is ARSB ELISA Kit (Human) in stock?

ARSB ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

What is the shelf life of this product?

Shelf life and expiration dates vary by product and are shown on the product listing and label. Products used beyond their expiration date are not eligible for performance-related returns or refunds, so please check the lot number and expiry before use.

What expression system was used for this recombinant protein?

The expression system, such as E. coli, HEK293, or insect cells, is listed on the datasheet under production details, which can affect post-translational modifications relevant to your application.

Can I return this product if I ordered the wrong one?

As a consumer, you have a 14-day right of withdrawal from receipt under EU consumer protection law, submitted via our withdrawal/cancellation request form. Note this applies to unused, undamaged products, and doesn’t override the expiry/storage conditions on performance-based claims.

ARSB ELISA Kit (Human)

Product Specifications

Reproducibility

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level ARSB were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level ARSB were tested on 3 different plates, 8 replicates in each plate. Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV Inter-Assay: CV

CAS Number

7732-18-5

Gene Name

Arylsulfatase B

Gene Aliases

Arylsulfatase B; ASB; G4S; MPS6; N-acetylgalactosamine-4-sulfatase.

Gene ID

411

Accession Number

NP_000037.2

Reactivity

Homo sapiens|Human

Target

Removes sulfate groups from chondroitin-4-sulfate (C4S) and regulates its degradation (PubMed:19306108) . Involved in the regulation of cell adhesion, cell migration and invasion in colonic epithelium (PubMed:19306108) . In the central nervous system, is a regulator of neurite outgrowth and neuronal plasticity, acting through the control of sulfate glycosaminoglycans and neurocan levels (By similarity) .

Type

ELISA Kit

Applications

Enzyme-linked immunosorbent assay

Assay Principle

Aviva Systems Biology ARSB ELISA Kit (Human) (OKDD00133) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for ARSB has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ARSB is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample ARSB captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell lysates

Detection Range

0.156-10ng/mL

Sensitivity

< 0.059 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

60 kDa

Notes

Formerly GWB-KBBZU3

Specificity

This assay has high sensitivity and excellent specificity for detection of ARSB. No significant cross-reactivity or interference between ARSB and analogues was observed.