What is the catalog number for GRN Chemi-Luminescent ELISA Kit (Rat)?

The catalog number for GRN Chemi-Luminescent ELISA Kit (Rat) is OKCD03540-96W.

What sizes are available for GRN Chemi-Luminescent ELISA Kit (Rat)?

GRN Chemi-Luminescent ELISA Kit (Rat) is available in the following size(s): 96 Well.

Is GRN Chemi-Luminescent ELISA Kit (Rat) in stock?

GRN Chemi-Luminescent ELISA Kit (Rat) is currently made to order / not held in immediate stock. Contact us for lead time.

Is this product species cross-reactive?

Cross-reactivity data, based on available testing, is listed under the reactivity section of the datasheet. Contact us if you need reactivity confirmed for a species not listed.

Is there an additional fee for hazardous or dry ice shipping of this product?

Yes, if this product requires dry ice or is classed as hazardous for transport, the additional fee will be explicitly shown in your quotation before you confirm the order, so there are no surprise charges later.

How many freeze-thaw cycles can this product tolerate?

We recommend aliquoting the product upon receipt to avoid repeated freeze-thaw cycles, which can reduce activity and stability. This is your responsibility once the product has been delivered.

GRN Chemi-Luminescent ELISA Kit (Rat)

Product Specifications

Reproducibility

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Granulin (GRN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Granulin (GRN) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

CAS Number

7732-18-5

Gene Name

Granulin precursor

Gene Aliases

Acrogranin; epithelin; granulins; PEPI; PGRN; proepithelin; progranulin; prostate cancer cell-derived growth factor.

Gene ID

29143

Accession Number

NP_058809

Reactivity

Rat|Rattus norvegicus

Target

Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling.

Type

ELISA Kit

Applications

Chemiluminescent immunoassay

Detection Method

Chemiluminescent

Assay Principle

The microplate provided in this kit has been pre-coated with an antibody specific to Granulin (GRN) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Granulin (GRN) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Granulin (GRN) level in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.14-100ng/mL

Recovery

Matrix Recovery range (%) Average (%) Serum (n=5) 95-102 98 EDTA Plasma (n=5) 91-99 94 Heparin Plasma (n=5) 93-105 102 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 95-102 98 Serum (n=5) 95-102 98 EDTA Plasma (n=5) 91-99 94 EDTA Plasma (n=5) 91-99 94 Heparin Plasma (n=5) 93-105 102 Heparin Plasma (n=5) 93-105 102

Sensitivity

1.56 ng/mL

Reconstitution

2°C to 8°C|-20°C

Components

Component Amount Grn Microplate 96 Wells (12 x 8 Well strips) Grn Lyophilized Standard 2 100X Grn Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Component Amount Component Amount Grn Microplate 96 Wells (12 x 8 Well strips) Grn Microplate 96 Wells (12 x 8 Well strips) Grn Lyophilized Standard 2 Grn Lyophilized Standard 2 100X Grn Biotinylated Detector Anitbody 1 x 120 uL 100X Grn Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Substrate Diluent 1 x 20 mL