QuicKey Pro Mouse CLU (Clusterin) ELISA Kit
Product Specifications
Abbreviation
CLU
UniProt
Q06890
Cross Reactivity
This kit recognizes Mouse CCL6 in samples. No significant cross-reactivity or interference between Mouse CCL6 and analogues was observed.
Target
APO-J; APOJ; CLI; KUB1; NA1/NA2; SGP2; SP-40; TRPM2
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cancer; Cardiovascular; Metabolism; Immunology; Signal transduction
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CLU. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse CLU are added to the micro ELISA plate wells. Mouse CLU in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Mouse CLU.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Mouse CLU. The concentration of Mouse CLU in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
1 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.31-20 ng/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
0.19 ng/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,6 months
Species
Mouse
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Mouse CLU concentrations in serum, plasma and other biological fluids.
Available Sizes
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