RuBisCO ELISA Kit (Plant)
Product Specifications
Reproducibility
Mean Intra-Assay Precision %CV: < 10% (n = 20) Mean Inter-Assay Precision %CV: < 20% (n = 20)
CAS Number
7732-18-5
Reactivity
Plant
Type
ELISA Kit
Assay Principle
Aviva Systems Biology RuBisCO ELISA Kit (Plant) (OKCA00374) is based on a competitive enzyme immuno assay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for RuBisCO and Horseradish Peroxidase (HRP) conjugated RuBisCO. The competitive inhibition reaction is launched between with HRP labeled RuBisCO and unlabeled RuBisCO with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of RuBisCO in the sample. The color development is stopped and the intensity of the color is measured.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Plant tissues
Detection Range
3.13 ug/mL - 800 ug/mL
Recovery
Sample 1 Sample Type Average Recovery Range leaf tissue (n=5) 0.95 89-103% Sample 1 Sample 1 Sample Type Average Recovery Range Sample Type Average Recovery Range leaf tissue (n=5) 0.95 89-103% leaf tissue (n=5) 0.95 89-103%
Sensitivity
< 3.13 ug/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
RuBisCO
Protein Name
RuBisCO
Gene Name URL
RuBisCO
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