LNG ELISA Kit

Sample Levonorgestrel Conc. (pg/mL) %CV 1 63.5 5.3 2 26.7 7.1 3 10.6 14.6 Sample Levonorgestrel Conc. (pg/mL) %CV Sample Levonorgestrel Conc. (pg/mL) %CV 1 63.5 5.3 1 63.5 5.3 2 26.7 7.1 2 26.7 7.1 3 10.6 14.6 3 10.6 14.6 Sample Levonorgestrel Conc. (pg/mL) %CV 1 59.9 6.8 2 27.2 7.8 3 9.0 12.6 Sample Levonorgestrel Conc. (pg/mL) %CV Sample Levonorgestrel Conc. (pg/mL) %CV 1 59.9 6.8 1 59.9 6.8 2 27.2 7.8 2 27.2 7.8 3 9.0 12.6 3 9.0 12.6

The DetectX® Levonorgestrel (LNG) Immunoassay kit is designed to quantitatively measure Levonorgestrel present in serum, plasma, saliva, urine, dried fecal samples, milk, and tissue culture media samples. Please read the complete kit insert before performing this assay. This kit measures total LNG in extracted serum, plasma, milk and fecal samples. A levonorgestrel stock solution is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. A levonorgestrel-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a rabbit polyclonal antibody to levonorgestrel to each well. After an hour incubation the plate is washed and substrate is added. The substrate reacts with the bound levonorgestrel-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the levonorgestrel in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Component Quantity Coated Clear 96 Well Plates 1 or 5 Each (-) -Levonorgestrel Standard 70 uL or 350 uL DetectX® Levonorgestrel Antibody 3 mL or 13 mL DetectX® Levonorgestrel Conjugate 3 mL or 13 mL Assay Buffer Concentrate 28 mL or 55 mL Wash Buffer Concentrate 30 mL or 125 mL TMB Substrate 11 mL or 55 mL Stop Solution 5 mL or 25 mL Plate Sealer 1 or 5 Each Component Quantity Component Quantity Coated Clear 96 Well Plates 1 or 5 Each Coated Clear 96 Well Plates 1 or 5 Each (-) -Levonorgestrel Standard 70 uL or 350 uL (-) -Levonorgestrel Standard 70 uL or 350 uL DetectX® Levonorgestrel Antibody 3 mL or 13 mL DetectX® Levonorgestrel Antibody 3 mL or 13 mL DetectX® Levonorgestrel Conjugate 3 mL or 13 mL DetectX® Levonorgestrel Conjugate 3 mL or 13 mL Assay Buffer Concentrate 28 mL or 55 mL Assay Buffer Concentrate 28 mL or 55 mL Wash Buffer Concentrate 30 mL or 125 mL Wash Buffer Concentrate 30 mL or 125 mL TMB Substrate 11 mL or 55 mL TMB Substrate 11 mL or 55 mL Stop Solution 5 mL or 25 mL Stop Solution 5 mL or 25 mL Plate Sealer 1 or 5 Each Plate Sealer 1 or 5 Each