POLR2J Antibody
Product Specifications
CAS Number
9007-83-4
Gene Name
RNA polymerase II subunit J
Gene Aliases
DNA-directed RNA polymerase II subunit J-1; DNA-directed RNA polymerase II subunit RPB11-a; hRPB14; POLR2J1; polymerase (RNA) II (DNA directed) polypeptide J, 13.3kDa; polymerase (RNA) II subunit J; RNA polymerase II 13.3 kDa subunit; RNA polymerase II subunit B11-a; RPB11; RPB11A; RPB11m.
Gene ID
5439
Swiss Prot
P52435
Accession Number
NP_006225.1
Reactivity
Human|Mouse
Immunogen
The antiserum was produced against synthesized peptide derived from human RPB11.
Target
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB11 is part of the core element with the central large cleft (By similarity) .
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
Enzyme-linked immunosorbent assay|Western blot
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Dilution
The optimal working dilution should be determined by the end user.
Format
Liquid
Reconstitution
-20°C
Molecular Weight
13 kDa
Notes
Formerly GWB-ASE541
Specificity
POLR2J antibody detects endogenous levels of total POLR2J protein.
NCBI Gene Symbol
POLR2J
Host or Source
Rabbit
Protein Name
DNA-directed RNA polymerase II subunit RPB11-a
Gene Name URL
POLR2J
Frequently Asked Questions
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