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BRAF Antibody (Phospho-Thr598)

Product Specifications

CAS Number

9007-83-4

Gene Name

B-Raf proto-oncogene, serine/threonine kinase

Gene Aliases

94 kDa B-raf protein; B-raf; B-Raf proto-oncogene serine/threonine-protein kinase (p94) ; B-Raf serine/threonine-protein; BRAF1; B-RAF1; murine sarcoma viral (v-raf) oncogene homolog B1; NS7; p94; proto-oncogene B-Raf; RAFB1; serine/threonine-protein kinase B-raf; v-raf murine sarcoma viral oncogene homolog B; v-raf murine sarcoma viral oncogene homolog B1.

Gene ID

673

Swiss Prot

P15056

Accession Number

NP_004324.2

Reactivity

Human|Mouse|Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human B-RAF around the phosphorylation site of Thr599.

Target

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Dilution

The optimal working dilution should be determined by the end user.

Format

Liquid

Reconstitution

-20°C

Molecular Weight

84 kDa

Notes

Formerly GWB-ASB327

Specificity

B-Raf (Phospho-Thr598) antibody detects endogenous levels of B-Raf only when phosphorylated at threonine 598.

NCBI Gene Symbol

BRAF

Host or Source

Rabbit

Protein Name

Serine/threonine-protein kinase B-raf

Gene Name URL

BRAF

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