Anti-Ki-67 Antibody

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable). {UniProtKB:E9PVX6, PubMed:10878551, PubMed:24867636, PubMed:27362226}.

<br><br>Immunohistochemistry: use at a dilution of 1:100-1:200 on formalin-fixed, paraffin-embedded samples after heat-induced epitope retrieval at pH 9 for 10-30 minutes.

Ki-67 is a nuclear, non-histone protein that is expressed only during active phases of the cell cycle (G1, S, G2, and M), but not in the resting phases of G0 and G1 early phase. Although it has been associated with ribosomal RNA transcription, it is strongly linked to cell proliferation and has been used as an effective marker in grading proliferation rate of tumors including those of brain, breast, cervix, and prostate.