Sulfo-NHS-SS-Biotin

KEY FEATURES: • Sulfo-NHS-SS-Biotin is amine-reactive and • Has a long chain spacer arm (24.3 Å) separating the biotin group from the amino-reactive sulfo-NHS ester • Water-soluble; can be used under physiologic conditions • It is possible to cleave the biotin group from the cross-linked target protein under reducing conditions, such as in the presence of BME, DTT or TCEP APPLICATIONS: • Sulfo-NHS-SS-Biotin is ideal for biotinylating antibody or protein that can then be used for protein purification, detection or immobilization • Sulfo-NHS-Biotin is particularly useful for targeting cell surface proteins due to the membrane-impermeability of this reagent