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Estrogen Receptor alpha Antibody

Estrogen receptor (ER) is an important regulator of growth and differentiation in the mammary gland. ER presence in breast tumors indicates an increased likelihood of response to anti-estrogen therapy.

Product Specifications

UniProt

P03372

Reactivity

Human

Immunogen

A recombinant human ESR1 protein fragment within amino acids 129-312 was used as the immunogen for the Estrogen Receptor alpha antibody.

Clonality

Monoclonal

Clone

NR3Ga-4

Conjugation

Unconjugated

Purification

Protein G affinity chromatography

Dilution

Immunofluorescence: 1-2ug/ml, Flow cytometry: 1-2ug/10^6 cells, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the Estrogen Receptor alpha antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

FACS, IF, IHC-P

Host or Source

Mouse

Preservative

1 mg/ml in 1X PBS; rAlbumin free, sodium azide free

Isotype

Mouse IgG1, kappa

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