Recombinant Human Interleukin-3 (IL3) (Active)
Product Specifications
Product Name Alternative
Interleukin-3; IL-3; Hematopoietic growth factor; Mast cell growth factor (MCGF) ; Multipotential colony-stimulating factor; P-cell-stimulating factor; IL3
Abbreviation
Recombinant Human IL3 protein (Active)
Gene Name
IL3
UniProt
P08700
Expression Region
20-152aa
Organism
Homo sapiens (Human)
Target Sequence
APMTQTTPLKTSWVNCSNMIDEIITHLKQPPLPLLDFNNLNGEDQDILMENNLRRPNLEAFNRAVKSLQNASAIESILKNLLPCLPLATAAPTRHPIHIKDGDWNEFRRKLTFYLKTLENAQAQQTTLSLAIF
Tag
C-terminal 6xHis-tagged
Type
Active Protein & In Stock Protein
Source
E.coli
Field of Research
Immunology
Relevance
Cytokine secreted predominantly by activated T-lymphocytes as well as mast cells and osteoblastic cells that controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells. Stimulates also mature basophils, eosinophils, and monocytes to become functionally activated. In addition, plays an important role in neural cell proliferation and survival.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Purity
Greater than 95% as determined by SDS-PAGE.
Activity
Yes
Bioactivity
The ED50 as determined by the dose-dependent stimulation of the proliferation of TF-1 cells is 5.972-17.16 ng/mL.
Form
Lyophilized powder
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Molecular Weight
22.0 kDa
References & Citations
Distinct Assemblies of Heterodimeric Cytokine Receptors Govern Stemness Programs in Leukemia. Kan W.L., Dhagat U., Kaufmann K.B., Hercus T.R., Nero T.L., Zeng A.G.X., Toubia J., Barry E.F., Broughton S.E., Lopez A.F. Cancer Discov 13:1922-1947 (2023)
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Full Length of Mature Protein
Available Sizes
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