Recombinant Acetobacter pasteurianus Modification methylase ApaLI (apaLIM)

Product Specifications

Product Name Alternative

Cytosine-specific methyltransferase ApaLI

Abbreviation

Recombinant Acetobacter pasteurianus apaLIM protein

Gene Name

ApaLIM

UniProt

O52702

Expression Region

1-429aa

Organism

Acetobacter pasteurianus (Acetobacter turbidans)

Target Sequence

MNKDEVVVSLFAGAGGFSSGFSQAGLKPLFGAEINADACQTYQENVGSPCHQLDLSTVDPSHIEMLTGGKRPFVVIGGPPCQGFSTAGPRNFADPRNLLIFNYLNIVERLSPRWLIFENVEGLLTSGGGRDLARLVREFVDMGYSVRLQKVNLAAYGVPQTRKRVLIIGNRLGIDFQFPEELYSFDSGKAKKASGKPLAPSLAEAVAGLGPAASDKDALVPYASSEPVNAFDARMRAGNRVEVVTHHVRVEAAERMQVELLKPGQTMKDLPPELWHESYRRRANRRVSDGTPTEKRGGAPSGIKRLHGNLQSLTITGPAAREFIHPTEHRPLTIRECARIQTFPDKYRWVGNNASVIQQIGNAVPPLAAERLAKHLRDIDGSFGADTRPAGAMSAKLLGFVLTEALGMSPALKSTEALLAEMHQGGFVF

Tag

N-terminal 6xHis-tagged

Type

Developed Protein

Source

Yeast

Field of Research

Microbiology

Relevance

This methylase recognizes the double-stranded sequence GTGCAC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the ApaLI endonuclease.

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Function

This methylase recognizes the double-stranded sequence GTGCAC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the ApaLI endonuclease.

Molecular Weight

48.5 kDa

References & Citations

"Cloning and expression of the ApaLI, NspI, NspHI, SacI, ScaI, and SapI restriction-modification systems in Escherichia coli."Xu S.-Y., Xiao J.-P., Ettwiller L., Holden M., Aliotta J., Poh C.L., Dalton M., Robinson D.P., Petronzio T.R., Moran L., Ganatra M., Ware J., Slatko B., Benner J. II Mol. Gen. Genet. 260:226-231 (1998)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.