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Recombinant Rat Glial Cell-derived Neurotrophic Factor

Product Specifications

Background

Glial cell-derived neurotrophic factor is a founding member of the GDNF family of ligands (GFL) and has been shown to interact with GFRA2 and GDNF family receptor alpha 1. It is a small protein that potently promotes the survival and morphological differentiation of various neuronal. It may also modulate local neuronal effects in distal regions of the motor neuron. GDNF Recombinant rat GDNF (monomer) contains 135 amino acids residues, which is a disulfide-linked homodimer and it shares 99 % and 93 % a.a. sequence identity with mouse and human GDNF.

Product Name Alternative

ATF

Gene ID

25453

UniProt

Q07731

Target

SPDKQAAALP RRERNRQAAA ASPENSRGKG RRGQRGKNRG CVLTAIHLNV TDLGLGYETK EELIFRYCSG SCEAAETMYD KILKNLSRSR RLTSDKVGQA CCRPVAFDDD LSFLDDSLVY HILRKHSAKR CGCI

Purification

> 98 % by SDS-PAGE and HPLC analyses.

Endotoxin

Less than 0.1 EU/ug of rRtGDNF as determined by LAL method.

Bioactivity

6

Storage Conditions

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculated Molecular Weight

Approximately 29.8 kDa, a homodimeric protein consisting of two 134 amino acid non-glycosylated polypeptide chains.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 1 x PBS, pH 7.4.

Host or Source

Escherichia coli

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