SirT1 (phospho-Ser27) rabbit pAb

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008],

Product Specifications

Background

UniProt

Q96EB6

Swiss Prot

Q96EB6

Reactivity

Human; Rat; Mouse

Immunogen

Synthesized phosho peptide around human SirT1 (Ser27)

Clonality

Polyclonal

Source

Rabbit

Applications

WB; ELISA; IHC

Concentration

1 mg/ml

Dilution

WB 1:500-2000; IHC-p 1:50-300; ELISA 2000-20000

Molecular Weight

85-110kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

85-110kD

Fragment

IgG

Subcellular Location

Nucleus, PML body . Cytoplasm . Nucleus . Recruited to the nuclear bodies via its interaction with PML (PubMed:12006491) . Colocalized with APEX1 in the nucleus (PubMed:19934257) . May be found in nucleolus, nuclear euchromatin, heterochromatin and inner membrane (PubMed:15469825) . Shuttles between nucleus and cytoplasm (By similarity) . Colocalizes in the nucleus with XBP1 isoform 2 (PubMed:20955178) . .; [SirtT1 75 kDa fragment]: Cytoplasm . Mitochondrion .

Other Product Names

NAD-dependent protein deacetylase sirtuin-1 (hSIRT1) (EC 3.5.1.-) (Regulatory protein SIR2 homolog 1) (SIR2-like protein 1) (hSIR2) [Cleaved into: SirtT1 75 kDa fragment (75SirT1) ]