CD16/CD32 mAb (2.4G2), InVivoPure+
CD16/CD32 (2.4G2) rat antibody recognizes endogenous levels of total CD16/CD32 protein. This antibody detects an epitope within the extracellular domain and is expected to detect all isoforms of CD16 and CD32. 2.4G2 antibody blocks the interaction between IgG and CD16 or CD32, and therefore is used as a pre-treatment to minimize non-specific binding of antibodies to FcR-bearing cells. CD16/CD32 is expressed on NK cells, monocytes, macrophages, dendritic C (at low levels) kupper cells, granulocytes, mast cells, B lymphocytesand some activated T lymphocyte
Product Specifications
Product Name Alternative
UniProt
P08101, P08508
Reactivity
Mouse
Immunogen
Animals were immunized with the J774 mouse macrophage cell line.
Target
CD16/CD32
Clonality
Monoclonal
Clone
2.4G2
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Concentration
≥ 1 mg/ mL
Purity
≥ 95 % (CGE, reducing conditions) ≤ 5 % aggregates (analytical SEC) Endotoxin level ≤ 1 EU/mg
Form
Clear Liquid, PBS, pH 7.4, 0.2 μm sterile filtered
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Recomended Isotype: Rat IgG2b Isotype control, Recomended Isotype Catalog: orb3127216
Host or Source
Rat
Preservative
Clear Liquid, PBS, pH 7.4, 0.2 μm sterile filtered
Isotype
Rat IgG2b k
Available Sizes
Frequently Asked Questions
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