Recombinant Mouse PDGF-BB

Product Specifications

Product Name Alternative

PDGFBB, PDGF-BB

UniProt

AAH53430.1

Expression Region

82-190

Host

E.Coli

Tag

C-terminal His Tag

Applications

Western Blot, ELISA

Endotoxin

< 1 EU/µg as determined by LAL test.

Purity

Greater than 95% as determined by SDS-PAGE.

Activity

Measured in a cell proliferation assay using Balb/c3T3 mouse fibroblast cells. The ED50 for this effect is 38.7 ng/ml.

Form

Lyophilized powder

Reconstitution

Centrifuge the vial at 10000 rpm for 30 s before opening, reconstitute in 4 mM HCl to concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex.

Molecular Weight

15 kDa

Shipping Conditions

4°C with ice bag

Storage Conditions

The lyophilized protein is stable at -20 °C for up to 1 year. For extended storage, it is recommended to further dilute in working aliquots after reconstitution. The protein solution is stable at ≤ -20 °C for 3 months, or 2-7 days at 2-8 °C under sterile conditions.Avoid repeated freeze/thaw cycle.