Recombinant Mouse PDGF-BB
Product Specifications
Product Name Alternative
PDGFBB, PDGF-BB
UniProt
AAH53430.1
Expression Region
82-190
Host
E.Coli
Tag
C-terminal His Tag
Applications
Western Blot, ELISA
Endotoxin
< 1 EU/µg as determined by LAL test.
Purity
Greater than 95% as determined by SDS-PAGE.
Activity
Measured in a cell proliferation assay using Balb/c3T3 mouse fibroblast cells. The ED50 for this effect is 38.7 ng/ml.
Form
Lyophilized powder
Reconstitution
Centrifuge the vial at 10000 rpm for 30 s before opening, reconstitute in 4 mM HCl to concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex.
Molecular Weight
15 kDa
Shipping Conditions
4°C with ice bag
Storage Conditions
The lyophilized protein is stable at -20 °C for up to 1 year. For extended storage, it is recommended to further dilute in working aliquots after reconstitution. The protein solution is stable at ≤ -20 °C for 3 months, or 2-7 days at 2-8 °C under sterile conditions.Avoid repeated freeze/thaw cycle.
Formulation
Lyophilized from a 0.2 μm filtered solution of 4 mM HCl.
Organism Species
Mouse
Available Sizes
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