DNA Damage ELISA Kit

1. Prepare samples and controls in 1X Assay Buffer or buffer of choice (0.5 mL at a cell density of 5 x 105 – 1 x 106 cells/mL)., 2. Reconstitute Total ROS Green with 100 µL DMSO to produce a 500X stock concentrate., 3. Dilute 1:10 by adding 900 µL 1X Assay Buffer to the 500X stock Total ROS Green to produce a 50X working solution., 4. Add Total ROS Green to each sample at 1:50. For example, add 10 µL into 490 µL cultured cells., 5. Incubate 1 hour at 37°C., 6. Treat cells with test compounds for desired period of time. Keep cells protected from light., 7. Analyze with a flow cytometer. Total ROS Green excites at 490 nm and emits at 520 nm.