Nitric Oxide Colorimetric Detection Kit

1. Prepare samples and controls., 2. Reconstitute ThioBright Green with 500 µL DMSO to produce a 200X stock concentrate., 3. Add ThioBright Green to each sample at 1:200. For example, add 5 µL to 995 µL cultured cells., 4. Incubate 15-30 minutes., 5. Optional: Spin and pellet cells, remove supernatants, and gently resuspend in Assay Buffer or other isotonic buffer., 6. If desired, label with additional stains, such as Hoechst, Propidium Iodide, 7-AAD, or an antibody., 7. Analyze with a flow cytometer. ThioBright Green excites at 490 nm and emits at 525 nm.