Antigen-Down ELISA Development Kit

1. Prepare samples., 2. Create controls with CCCP., 3. Dilute 10X Assay Buffer 1:10 with diH2O., 4. Reconstitute MitoPT TMRE with DMSO., 5. Dilute MitoPT TMRE with 1X Assay Buffer., 6. Add MitoPT TMRE to each sample., 7. Incubate 15-30 minutes., 8. Wash cells: add 1X Assay Buffer and spin cells., 9. Remove supernatant and resuspend cells for analysis., 10. Anazlyze with a fluorescence microscope, fluorescence plate reader, or flow cytometer. MitoPT TMRE excites at 549 nm and emits at 574 nm (orange).