General Serum Diluent, 2X

1. Dilute the standard curve, controls, and the samples as necessary. ICT offers several formulations of sample diluents in which to prepare the samples., 2. Prepare 1X working strength assay diluent by diluting 1:2 in diH2O. Add 1 part General Assay Diluent, 2X to 1 part diH2O. Mix well., 3. Pipette 50-100 μL General Assay Diluent per well into every well of the plate., 4. Pipette 50-200 μL of each standard, control, and sample into the plate., 5. Run the assay according to the specific ELISA protocol., 6. Analyze the data. Because all of the wells, including the standards and controls, received the same volume of assay diluent, there is no need to account for this dilution when calculating the results.