General Serum Diluent, 2X
2X General Serum Diluent is formulated for diluting goat, rabbit, mouse, avian, and human sera, as well as mouse ascites fluid and cell culture media, for testing in sandwich ELISA configurations. It can also be used for dilution of many types of serum samples in antigen-down ELISA formats. Our 2X concentrated version is helpful for shipping purposes and produces less waste.
Product Specifications
Assay Principle
1. Dilute the standard curve, controls, and the samples as necessary. ICT offers several formulations of sample diluents in which to prepare the samples., 2. Prepare 1X working strength assay diluent by diluting 1:2 in diH2O. Add 1 part General Assay Diluent, 2X to 1 part diH2O. Mix well., 3. Pipette 50-100 μL General Assay Diluent per well into every well of the plate., 4. Pipette 50-200 μL of each standard, control, and sample into the plate., 5. Run the assay according to the specific ELISA protocol., 6. Analyze the data. Because all of the wells, including the standards and controls, received the same volume of assay diluent, there is no need to account for this dilution when calculating the results.
pH
pH: 7.2-7.6
Shipping Conditions
Blue Ice
Storage Conditions
4°C
Shelf Life
24 months from date of manufacture
Frequently Asked Questions
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