Protein-Free Sample Diluent
Protein-Free Sample Diluent is a protein-free matrix used for the dilution of biological samples (e.g. serum, cell culture media) into the useful range of antibody-sandwich or antigen-down ELISA format assays. This unique buffer contains a heterogeneous mixture of proprietary molecules that help reduce background noise associated with non-specific bridging of signal-generating conjugates to the plate well surface.
Product Specifications
Assay Principle
1. Protect the HRP Conjugate Stabilizer from light. This may be done by covering the container with aluminum foil., 2. Add the HRP-conjugated protein at the target concentration to HRP Conjugate Stabilizer. For example, if diluting an HRP-conjugated antibody 1:5000, add 10 µL conjugate stock to 49.99 mL HRP Conjugate Stabilizer., 3. Mix for 15 minutes., 4. Use the diluted HRP conjugate in the immunoassay., 5. Store remaining HRP conjugate solution at 2-8°C. Protect from direct exposure to light.
pH
pH 7.1-7.6
Shipping Conditions
Blue Ice
Storage Conditions
4°C
Shelf Life
24 months from date of manufacture
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