Phosph-Free Block ELISA Blocking Buffer

Phosph-Free Block ELISA Blocking Buffer is a novel non-protein blocking formulation designed to eliminate interference and nonspecific background noise associated with antibody-coated ELISA formats and sandwich ELISAs. Phosph-Free Block is formulated for ELISAs using alkaline phosphatase detection or for assays with ultra-sensitivity requirements. Use of this blocking formulation provides superior background performance without the use of conventional cross-reactive protein additives.

Product Specifications

CAS Number

7732-18-5

Target Antigen

Hydrogen Peroxide

Assay Principle

1. Add the antibody-AP or protein-AP conjugate directly into the Alkaline Phosphatase Conjugate Stabilizer to a working 1X dilution and mix for 5-15 minutes., 2. Pipette diluted AP conjugate onto the plate or store protected from light at 2-8°C for use at a later date. 1X working AP conjugate dilutions can be stored at 2-8°C and protected from light providing a useful shelf-life of 6 months.