Phosph-Free Block ELISA Blocking Buffer
Phosph-Free Block ELISA Blocking Buffer is a novel non-protein blocking formulation designed to eliminate interference and nonspecific background noise associated with antibody-coated ELISA formats and sandwich ELISAs. Phosph-Free Block is formulated for ELISAs using alkaline phosphatase detection or for assays with ultra-sensitivity requirements. Use of this blocking formulation provides superior background performance without the use of conventional cross-reactive protein additives.
Product Specifications
Target Antigen
Hydrogen Peroxide
Assay Principle
1. Add the antibody-AP or protein-AP conjugate directly into the Alkaline Phosphatase Conjugate Stabilizer to a working 1X dilution and mix for 5-15 minutes., 2. Pipette diluted AP conjugate onto the plate or store protected from light at 2-8°C for use at a later date. 1X working AP conjugate dilutions can be stored at 2-8°C and protected from light providing a useful shelf-life of 6 months.
pH
pH: 7.4 at 1X
Shipping Conditions
Blue Ice
Storage Conditions
4°C
Shelf Life
24 months from date of manufacture
CAS Number
7732-18-5
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