Antigen-Down HRP Conjugate Stabilizer, 5X
Antigen-Down HRP Conjugate Stabilizer is a unique formulation specifically designed for use with anti-IgG-HRP conjugates. This stabilizing diluent contains proprietary non-mammalian protein additives that will not interfere with anti-IgG-HRP conjugates used in for antigen-down immunoassays or sandwich ELISA formats. These additives provide additional structural stability to the IgG-HRP conjugate protein complex. Antigen-Down HRP Conjugate Stabilizer will also inhibit conjugate binding to nonspecific serum proteins bound to the plate, reducing conjugate bridging on the plate surface and background noise.
Product Specifications
CAS Number
9007-83-4
Target Antigen
Apoptosis, Caspases 3/7
Assay Principle
When using HRP Conjugate Stock Stabilizer, 5X as a Conjugate Diluent:, 1. Add 1 part HRP Conjugate Stock Stabilizer, 5X to 4 parts deionized water and mix for 5-15 minutes., 2. Add the antibody-HRP or protein-HRP conjugate directly into the conjugate diluent to a working 1X dilution and mix for 5-15 minutes., 3. Pipette diluted conjugate onto the plate, or store protected from light at 2-8°C for use at a later date. 1X working conjugate dilutions can be stored at 2-8°C, protected from light, providing a useful shelf-life of 6-12 months., , When using HRP Conjugate Stock Stabilizer, 5X as a Concentrated Conjugate Stock Storage Buffer:, 1. To purified HRP conjugate, add an equal volume of the 5X HRP Conjugate Stock Stabilizer., 2. Filter sterilize into a sterile amber or brown-glass container., 3. Store at 2-8°C. Concentrated HRP conjugate stocks should be stable for up to 18 months.
pH
pH: 7.4 at 1X
Shipping Conditions
Blue Ice
Storage Conditions
4°C
Shelf Life
24 months from date of manufacture
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
