7-AAD Red Fluorescent Live/Dead Stain

7-Aminoactinomycin D (7-AAD) is a red fluorescent vital dye that intercalates in double-stranded DNA with a high affinity for GC-rich regions. 7-AAD does not pass through intact cell membranes. It may also be used to label all cells after fixation.

Product Specifications

Target Antigen

Apoptosis, Poly Caspases

Assay Principle

1. Add 1 part Antigen-Down HRP Conjugate Stabilizer to 4 parts deionized water and mix for 5-15 minutes., 2. Add the anti-IgG-HRP conjugate directly into the conjugate diluent to a working 1X dilution and mix for 5-15 minutes., 3. Pipette diluted conjugate onto the plate, or store protected from light at 2-8°C for use at a later date. 1X working conjugate dilutions can be stored at 2-8°C and protected from light, providing a useful shelf-life of 6-12 months.